radioprotective effects of selenium and vitamin-e against 6mv x-rays in human blood lymphocytes by micronucleus assay

نویسندگان

aram rostami department of medical physics, faculty of medicine, iran university of medical sciences, tehran, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (iran university of medical sciences)

seyed akbar moosavi department of lab sciences, school of allied medicine, iran university of medical sciences, tehran, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (iran university of medical sciences)

vahid changizi department of technology of radiology and radiotherapy, school of allied medicine, tehran university of medical sciences, tehran, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (tehran university of medical sciences)

ali abbasian ardakani department of medical physics, faculty of medicine, iran university of medical sciences, tehran, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (iran university of medical sciences)

چکیده

background: critical macromolecules of cells such as dna are in exposure to damage of free radicals that induced from the interaction of ionizing radiation with biological systems. selenium and vitamin-e are natural compounds that have been shown to be a direct free radical scavenger. the aim of this study was to investigate the radioprotective effect of selenium and vitamin-e separately and synergistically against genotoxicity induced by 6mv x-rays irradiation in blood lymphocytes.   methods: fifteen volunteers were divided into three groups include a, b and c. these groups were given selenium (800iu), vitamin-e (100mg) and selenium (400iu) + vitamin-e (50mg), respectively. peripheral blood samples were collected from each group before (0hr) and 1, 2 and 3hr after selenium and vitamin-e administration (separately and synergistically). then the blood samples were irradiated to 200cgy of 6mv x-rays. after that lymphocyte samples were cultured with mitogenic stimulation to determine the chromosomal aberrations with micronucleus assay in cytokinesis-blocked binucleated cells.   results: the lymphocytes in the blood samples collected at one hr after ingestion selenium and vitamin-e, exposed in vitro to x-rays exhibited a significant decrease in the incidence of micronuclei, compared with control group at 0hr. the maximum protection and decrease in frequency of micronuclei (50%) were observed at one hr after administration of selenium and vitamin-e synergistically.   conclusion: the data suggest that ingestion of selenium and vitamin-e as a radioprotector substance before exposures may reduce genetic damage caused by x-rays irradiation.

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Radioprotective effects of selenium and vitamin-E against 6MV X-rays in human blood lymphocytes by micronucleus assay

Background: Critical macromolecules of cells such as DNA are in exposure to damage of free radicals that induced from the interaction of ionizing radiation with biological systems. Selenium and vitamin-E are natural compounds that have been shown to be a direct free radical scavenger. The aim of this study was to investigate the radioprotective effect of selenium and vitamin-E separately and sy...

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Radioprotective effects of selenium and vitamin-E against 6MV X-rays in human blood lymphocytes by micronucleus assay

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عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۳۰، شماره ۱، صفحات ۴۶۹-۴۷۵

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